Chromophore Assignment in C-Phycocyanin from Mastigocladus laminosus
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چکیده
منابع مشابه
ZZE -Configuration of Chromophore ß-153* in C-Phycocyanin from Mastigocladus laminosus
The photochemistry of C-phycocyanin has been studied after denaturation in the dark. It shows an irreversible reaction which has characteristics of a Z .Z .E to Z ,Z,Z-isom erization of dihydrobilins. Its amplitude depends on the reaction conditions, with a maximum corresponding to 15% conversion of one of the three PC chromophores. This chromophore is suggested to be ß-153, for which recent X-...
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The behaviour o f monomers and trimers o f C-phycocyanin and allophycocyanin under high pressure is studied by UV-VIS absorption spectroscopy. It is found that fast variations o f pres sure (Ap > 500 bar) and/or temperature are accompanied by significant changes in the absorp tion spectra (intensity and/or spectral shift). The induced differences disappear, however, in part, when the samples ...
متن کاملPICOSECOND TIME-RESOLVED FLUORESCENCE OF PHYCOBILIPROTEINS: SUBUNITS OF PHYCOCYANIN FROM Mastigocladus laminosus
The (Yand &subunits of C-phycocyanin from Mastigocladus laminosus were prepared according to revised procedures. Both subunits are isolated as dimers, which can be dissociated into monomers with detergent mixtures. The fluorescence decay kinetics are similar for the respective monomers and dimers. In no case could they be fitted by only one (o-subunit) or two exponentials (P-subunit) which are ...
متن کاملPhylogeography of the thermophilic cyanobacterium Mastigocladus laminosus.
We have taken a phylogeographic approach to investigate the demographic and evolutionary processes that have shaped the geographic patterns of genetic diversity for a sample of isolates of the cosmopolitan thermophilic cyanobacterial Mastigocladus laminosus morphotype collected from throughout most of its range. Although M. laminosus is found in thermal areas throughout the world, our observati...
متن کاملRECONSTITUTION OF ALLOPHYCOCYANIN FROM Mastigocladus laminosus WITH ISOLATED LINKER POLYPEPTIDE
The core linker polypeptide L,8 was isolated from Mastigocladus laminosus and purified on a preparative scale. A method for the reconstitution of allophycocyanin (Aptlinker complexes from isolated polypeptides was developed. The complex (aA“(3AP)3 .L,8.9 was reconstituted and compared to (aAP(3AP) and (aAppAP)3 by sucrose density gradient ultracentrifugation, absorption, fluorescence emission a...
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ژورنال
عنوان ژورنال: Zeitschrift für Naturforschung C
سال: 1987
ISSN: 1865-7125,0939-5075
DOI: 10.1515/znc-1987-0315